cell clone造句

• Recent progress in handmade somatic cell cloning
  徒手切割法体细胞克隆研究进展
• The es cell clones decreased and disappeared gradually after 9 passages
  本实验中的小鼠胚胎es细胞再传至第九代后逐渐消失。
• In terms of human cell cloning , he gained a great deal of experience
  在人体活细胞（克隆）培养增殖技术方面积累了丰富的经验。
• Plasma cell clone
  浆细胞纯系
• Of more immediate concern is how telomere length might affect human cells cloned to help fight diseases , rather than cloning an entire human
  目前更紧迫的问题不是克隆人，而是要搞清端粒长度如何影响克隆的人体细胞，从而帮助治疗疾病。
• The plasmid ploxgfp dna was transfected into bovine fetal fibroblast cells , then on the selected g418 medium , the green fluorescent cell clones were gained
  将鸡-干扰素cdna克隆到载体plox上的loxp和lox511序列之间，构建成另一打靶载体? ploxifn 。
• ( 4 ) the mrna and protein expression grp94 were detected by rt - pcr and western bloting respectively to find the cell clones that have been transfected successfully . 2
  ( 4 )分别通过rt - pcr方法和westernblot方法从mrna和蛋白水平检测grp94的表达情况，以此鉴定转染成功的阳性克隆株。
• There was about 7 days interval between every two passages . eg cell clones can be found in each primary culture from those embryos of appropriate age . in culture of one embryonic cell line , eg cell clones maintained after 9 passages
  在实验中，几个适龄的胚胎的原代培养物中都出现了鸟巢状的eg细胞集落，维持时间最长的一个胚胎细胞株在传代培养了9代之后仍然可以观察到eg细胞集落。
• The two mediums can meet primary culture and passage culture of the black bear fibroblast cells . the method of single cell cloning by micro - manipulating purifies fibroblast cells . as a result , steady fibroblast cells can be obtained and the rates of the cloning formation is 93 . 75 %
  通过单细胞集落显微法纯化细胞，可克服单细胞克隆中因细胞密度太低，难以形成克隆的缺点，而且操作简单，效率高，速度快，其克隆率可达93 . 75 。
• The main contents and results were as follows : 1 . the establishment of sd rat fibroblast for the donor of nuclear transfer the method of single cell cloning by micro - manipulating was improved to purify fibroblast . as a result , steady fibroblast can be obtained and the rate of the clone formation is 92 . 71 %
  主要内容和结果如下： (一) sd大鼠成纤维细胞系的建立将单细胞显微法改进为单细胞集落克隆纯化，能快速、有效建立细胞系，并可获得92 . 71克隆率。
• M ethods we successfully expanded human embryonic brain - derived nsc into spheres with mitogens . the nsc were identified by immunocytochemistry method , brdu labeling and cell cloning were used to observe the proliferation ability of nsc . pdgf x t3 were separately used to induce the differetiation of nsc
  方法：本实验以胎龄为10 - 12周的人大脑皮质为材料，在体外成功诱导扩增nsc ，用免疫细胞化学方法鉴定nsc ，用brdu标记和细胞克隆分析观察了nsc的增殖能力。
• Finally , that effect of different culture mediums and matrix on the proliferation and differentiation of hpfl has investigated by mtt assay . 2 ) adopted micromanipulation cell cloning and immunohistochemistry staining , alb ck - 19 clones of hpfl were choosed and seeded in inducing medium to show whether the alb + ck - 19 + cells appear
  2 )将初步纯化的ed13 . 5d的大鼠hpfl采用显微法进行克隆培养，免疫组化法第四军医大学硕士学位论文筛选alb ” ck 19一的克隆进行诱导培养，观察alb ” ck 19一的细胞分化趋势。
• After the examination of pcr , one non - fluorescent cell clone had the same result as the plasmid ploxifn - a 1000bp product ; another non - fluorescent cell clone showed a 500bp product , a deletion reaction was thought to happen between the two lox sites in the genome under the reaction of cre recombinase so that the gfp gene and neo gene etc were cut
  经pcr检测后，有一个不发光细胞克隆扩增出了与对照质粒ploxifn相同的约1000bp的条带，表明发生了替换反应；另一个不发光细胞克隆认为是在cre重组酶的作用下其内部自行发生了剪切作用，剪切了gfp基因，从而只扩增出了载体序列和一个lox位点的约500bp的条带。
• The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue , then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells . after screeened with g418 and cloned , 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons . it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level
  抽提、鉴定、纯化重组质粒后，脂质体转染法将重组质粒导入中国仓鼠卵巢细胞( cho - dhfr ~ - )中， g418选择转染子并克隆化培养，经rt - pcr和分子灯塔探针杂交鉴定其mrna转录，获得4株稳定表达54位密码突变型mbl的cho细胞。
• After multiple , plasmid ploxifn and pbs185 dna were co - transfected into the green fluorescent cell clones , then filtrated the non - fluorescent cell clones . two non - fluorescent cell clones , the green fluorescent cell clones and the cells which were co - transfected by the plasmid ploxifn and pbsiss dna were checked up by pcr
  首先将质粒ploxgfpdna电转染牛胎儿成纤维细胞，并用g418筛选，筛选出绿色荧光细胞克隆，增殖培养之后，将质粒ploxifn和pbs185dna共转染荧光细胞克隆，筛选出不发光的克隆。
• Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months . the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg , and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa . through three clonings less diversed anti - p24 hybridoma cells are gained
  方法：基因工程p24抗原免疫小鼠4次，历时2个月，取脾细胞与骨髓瘤细胞株sp2 0 ，用peg融合， hat选择培养和间接elisa筛选分泌抗p24抗体阳性的杂交瘤细胞，三次克隆化后得稳定分泌抗p24抗体的杂交瘤细胞株。
• The blastocyst was obtain from the mouse after 3 . 5 days of pregnancy , and was cultivated on the mouse embryonic flbroblast feeder layer . the blastocyst usually attach to the feeder layer after 48 hours , then the inner cell mass began to grow and form a big cell mass of embryonic stem cells . these cells can form cell clones with the conformation of embryonic stem cell
  囊胚一般在48小时后贴壁并脱去透明带，由囊胚中的内细胞团增殖形成一个胚胎干细胞球，待其生长至一定程度后对其进行酶解传代，在传代后的细胞中重新生成es集落形态的细胞集落，在集落生长至一定程度后再进行传代，此后大约每5 ? 7天传代一次。
• Using the green fluorescent cell clones as the template , a product of 4kb which was the same as the result of pcr of the plasmid ploxgfp was got . on the other hand , not only a 4kb product but also a 1000bp product and a 500bp product were showed when the co - transfected cells were used to be the template
  以绿色荧光细胞克隆为模板扩增出了包括gfp基因、 neo基因等在内的约4kb的条带；以共转染后的未经筛选的细胞为模板既扩增出了约1000bp的条带，又扩增出了约4kb和500bp的条带。